A new murine model to study musculoskeletal tuberculosis (short communication)

作者：Kager Lie**eth M^*; Runge Jurgen H; Nederveen Aart J; Roelofs Joris J T H; Stoker Jaap; Maas Mario; van der Poll Tom

来源：Tuberculosis, 2014, 94(3): 306-310.

DOI：10.1016/j.tube.2014.01.002

• Bone and joint tuberculosis
• Spinal tuberculosis
• Pott's disease
• Magnetic resonance imaging
• Musculoskeletal imaging
• Mice

Musculoskeletal tuberculosis (TB) is a severe extrapulmonary manifestation of chronic Mycobacterium (M.) tuberculosis infection. Considering increasing incidence, multi-drug resistance and associated treatment difficulties, more preclinical research is needed. In this study we developed a murine model for musculoskeletal TB. Mice, intranasally infected with M. tuberculosis, were sacrificed after ten months. Mycobacterial growth was detected in lung and femur homogenates. Ziehl-Neelsen staining of paraffinembedded femurs showed acid-fast rods in the myelum and Magnetic Resonance Imaging demonstrated osteomyelitis and macronodular tuberculomas. This new murine model of musculoskeletal TB might be of value to further investigate immunologic and radiologic responses.

• 出版日期2014-5