A rhamnan-type sulfated polysaccharide with novel structure from Monostroma angicava Kjellm (Chlorophyta) and its bioactivity

作者：Liu, Xue; Hao, Jiejie^*; He, Xiaoxi; Wang, Shuyao; Cao, Sujian; Qin, Ling; Mao, Wenjun^*

来源：Carbohydrate Polymers, 2017, 173: 732-748.

DOI：10.1016/j.carbpol.2017.06.031

• Monostroma angicava Kjellm
• Sulfated polysaccharide
• Structure
• Antidiabetic activity
• Anticoagulant property

A homogeneous polysaccharide was obtained from Monostroma angicava Kjellm by water extraction, preparative anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that the polysaccharide was a glucuronic acid-containing rhamnan-type sulfated polysaccharide. The backbone mainly consisted of -> 3)-alpha-L-Rhap-(1 -> and -> 2)-alpha-L-Rhap-(1 -> residues, partially sulfated at C-2 of -> 3)-alpha-LRhap-(1 -> and C-3/C-4 of -> 2)-alpha-L-Rhap The branching contained unsulfated or monosulfated 3-linked, 2-linked, 4-linked alpha-L-rhamnose and terminal beta-D-glucuronic acid residues. The polysaccharide had strong antidiabetic activity assessed by glucose consumption, total cholesterol and triglyceride levels using human hepatocellular carcinoma (HepG2) and insulin-resistant HepG2 cells. The polysaccharide exhibited high anticoagulant property by activated partial thromboplastin time and thrombin time assays, and possessed high fibrin(ogen)olytic activity evaluated by plasminogen activator inhibitior-1, fibrin(ogen) degradation products and D-dimer levels using rats plasma. The investigation demonstrated that the polysaccharide from Monostroma angicava Kjellm was a novel sulfated rhamnan and could be a potential antidiabetic and anticoagulant polysaccharide.

• 出版日期2017-10-1
• 单位中国海洋大学