MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma

作者：Tian, Linli; Zhang, Jiarui; Ge, Jingchun; Xiao, Hui; Lu, Jianguang; Fu, Songbin; Liu, Ming^*; Sun, Yanan

来源：Medical Oncology, 2014, 31(1): 785.

DOI：10.1007/s12032-013-0785-3

• Laryngeal squamous cell carcinoma
• MiR-205
• Bcl-2
• Proliferation
• Apoptosis

MicroRNAs were reported to be involved in the modulation of tumor development. The aim of our study was to investigate the effect of miR-205 on proliferation and apoptosis of laryngeal squamous cell carcinoma (LSCC) and seek associations between miR-205 and Bcl-2 using in vitro and in vivo methods. Real-time qPCR was used to analyze the expression of miR-205 in LSCC samples and Hep-2 cell line. Apoptosis, cell cycle, and proliferation (MTT) assays were performed to test the apoptosis and proliferation of LSCC cells after miR-205 transfection. Bcl-2 expression in cells was assessed with Western blotting. The tumorigenicity of LSCC cells was evaluated in nude mice model. MiR-205 was significantly down-regulated in LSCC tissues compared to adjacent normal tissues. Lower expression of miR-205 was indicated to be statistically related with advanced clinical stage and T3-4 grades. We found that restoration of miR-205 down-regulated the proliferative markers of dihydrofolate reductase and proliferating cell nuclear antigen and apoptotic regulator of Bcl-2. The findings in vitro and in vivo showed miR-205 could suppress cell proliferation and induce cell apoptosis. In addition, Bcl-2 was identified as one of the direct targets of miR-205 in LSCC cells. These results suggest that miR-205 may play as a tumor suppressor in LSCC, probably by targeting Bcl-2 and serve as a potential target for therapeutic intervention.

• 出版日期2014-1
• 单位哈尔滨医科大学