Proteoliposomes from Mycobacterium smegmatis induce immune cross-reactivity against Mycobacterium tuberculosis antigens in mice

作者：Rodriguez Lissete; Tirado Yanelys; Reyes Fatima; Puig Alina; Kadir Ramlah; Borrero Reinier; Fernandez Sonsire; Reyes Giselle; Alvarez Nadine; Garcia Maria Angeles; Sarmiento Maria Elena; Norazmi Mohd Nor^*; Quinoy Jose Luis Perez; Acosta Armando

来源：Vaccine, 2011, 29(37): 6236-6241.

DOI：10.1016/j.vaccine.2011.06.077

• Antibody response
• Delayed type hypersensitivity
• Mycobacterium smegmatis
• M. tuberculosis
• Proteoliposomes

Proteoliposomes (PL) obtained from Mycobacterium smegmatis (Ms) were evaluated for their capacity to elicit cross-reactive responses against Mycobacterium tuberculosis (Mtb) antigens in BALB/c mice. Animals immunized with PL adjuvanted with alum (PL-AL) or Freund's Incomplete Adjuvant (PL-IFA) showed significant IgG responses against the PL as well as total Ms lipids. Both groups of animals also showed significant IgG responses against BCG, but only animals immunized with PL-AL produced significant IgG responses against soluble cell wall proteins (SCWP) or whole cell lysate (WCL) of Mtb. Significant DTH responses against WCL were observed in both groups of animals after 24 h, but only PL-AL-immunized mice showed significant DTH responses after 48 h and 72 h. PL-Ms are capable of eliciting cross-reactive humoral and cellular responses against Mtb antigens and thus may be a potential vaccine strategy against tuberculosis.

• 出版日期2011-8-26