A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus

作者：Jiang, Mengting; Luo, Xiangwen; Zhang, Deyong; Zhou, Xuguo; Ouyang, Xian; Zhang, Zhanhong; Li, Fan; Zhang, Songbai^*; Liu, Yong^*

来源：Canadian Journal of Plant Pathology, 2017, 39(2): 212-217.

DOI：10.1080/07060661.2017.1316774

• detection
• pepper
• Pepper vein yellows virus
• PeVYV
• RT-LAMP
• RT-PCR

Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62 degrees C for 45min and the whole reaction took less than 1h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.

• 出版日期2017
• 单位云南农业大学; 湖南农业大学; 湖南省农业科学院