Apoptotic death mode of mitomycin C-treated HeLa cells and cellular localization of mitomycin C-induced P-glycoprotein

作者：Cheng Min Hsiung; Cheng Hao Tsai; Lin Shih Shen; Young Su Chung; Pai Chien Jung; Liao Pao Hsin; Chen Shu Chen; Chou Ming Yung; Yang Jaw Ji; Yang Chi Chiang^*

来源：Drug and Chemical Toxicology, 2009, 32(2): 158-168.

DOI：10.1080/01480540802594491

• Apoptosis
• HeLa cell
• mitomycin C
• P-glycoprotein
• confocal microscopy

Mitomycin C (MMC) is an active antineoplastic agent and is suggested to induce apoptosis in a caspase-dependent manner in human gastric, bladder, and breast cancer cells. In this study, the death mode of human cervical cancer cells (HeLa) induced by MMC and the cellular localization of MMC-induced P-glycoprotein (P-gp) were investigated. The results of caspase-3 activity, Annexin V binding, and DNA fragmentation suggested that the degree of caspase-dependent apoptosis induced by MMC was in a dose-, but not time-dependent, manner. Further, in low-dose (0.0299 mu M) and long-term (2 months) treatment with MMC, P-gp is itself extruded from the cells and colocalized with nuclear DNA and the overexpression was achieved.

• 出版日期2009-4