Acinetobacter dijkshoorniae sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex mainly recovered from clinical samples in different countries

作者：Cosgaya Clara; Mari Almirall Marta; Van Assche Ado; Fernandez Orth Dietmar; Mosqueda Noraida; Telli Murat; Huys Geert; Higgins Paul G; Seifert Harald; Lievens Bart; Roca Ignasi; Vila Jordi

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(10): 4105-4111.

DOI：10.1099/ijsem.0.001318

The recent advances in bacterial species identification methods have led to the rapid taxonomic diversification of the genus Acinetobacter. In the present study, phenotypic and molecular methods have been used to determine the taxonomic position of a group of 12 genotypically distinct strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, initially described by Gerner-Smidt and Tjernberg in 1993, that are closely related to Acinetobacter pittii. Strains characterized in this study originated mostly from human samples obtained in different countries over a period of 15 years. rpoB gene sequences and multilocus sequence typing were used for comparisons against 94 strains representing all species included in the ACB complex. Cluster analysis based on such sequences showed that all 12 strains grouped together in a distinct clade closest to Acinetobacter pittiithat was supported by bootstrap values of 99 %. Values of average nucleotide identity based on BLAST between the genome sequence of strain JVAP01(T) (NCBI accession no. LJPG00000000) and those of other species from the ACB complex were always <91.2 %, supporting the species status of the group. In addition, the metabolic characteristics of the group matched those of the ACB complex and the analysis of their protein signatures by matrix-assisted laser desorption ionization time-of-flight MS identified some specific peaks. Our results support the designation of these strains as representing a novel species, for which the name Acinetobacter dijkshoorniae sp. nov. is proposed. The type strain is JVAP01(T) (= CECT 9134(T) = LMG 29605(T)).

• 出版日期2016-10
• 单位河北医科大学