Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay

作者：Dalby Tine^*; Sorensen Charlotte; Petersen Jesper Westphal; Krogfelt Karen Angeliki

来源：Acta Pathologica, Microbiologica et Immunologica Scandinavica, 2010, 118(12): 968-972.

DOI：10.1111/j.1600-0463.2010.02664.x

• Bordetella pertussis
• ELISA
• antibody
• human

Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or with heat-treated sera (IgG anti-PT ELISA). These two improved techniques were compared by the analysis of 100 individual serum samples from a previous clinical trial and 213 sera from a longitudinal serum collection from 20 Danish adults recently vaccinated with the Danish acellular pertussis vaccine. The comparison showed a significant linear correlation between the results of the two assays with a p-value of < 0.0001 for the 100 individual samples. We, therefore, conclude that the improved IgG anti-PT ELISA can be used as a replacement for the often troublesome and time-consuming CHO cell assay for the measurement of vaccine-induced human antibodies to PT.

• 出版日期2010-12