Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass

作者：Hanada Yasutaka^*; Sugioka Koji; Kawano Hiroyuki; Ishikawa Ikuko Shihira; Miyawaki Atsushi; Midorikawa Katsumi

来源：Biomedical Microdevices, 2008, 10(3): 403-410.

DOI：10.1007/s10544-007-9149-0

• nano-aquarium
• microchip
• microorganism
• living cell
• femtosecond laser
• photostructurable glass

We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms and cells in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms and cells.

• 出版日期2008-6