科研之友
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摘要
In this work, we report a novel sandwich-type electrochemical aptasensor for sensitive and selective detection of antibiotic kanamycin. This aptasensor was constructed by assembling graphene-polyanitine (GR-PANI) and potyamidoamine dendrimer-Au nanoparticte (PAMAM-Au) nanocomposites on the surfaces of a glass carbon electrode, which as electrode materials exhibited excellent charge-transport property and loading capacity of biomolecules. Followed by the sandwich-type specific identification, kanamycin detection was realized by outputting a redox current from electro-reduction reaction of H2O2 catalyzed by HRP. It was demonstrated that the developed strategy had a wide linear response range from 5 x 10(-6) to 4 x 10(-2) mu g mL(-1) and a low detection limit of 4.6 x 10(-6) mu g mL(-1). Additionally, this aptasensor had the advantages in its low cost, high sensitivity, and superb specificity. Thus, it might provide a useful tool for kanamycin determination and related food safety analysis and clinical diagnosis.
