DEVELOPMENT OF HETEROLOGOUS ENZYME LINKED IMMUNOSORBENT ASSAY FOR DEHYDROEPIANDROSTERONE IN SERUM

作者：Shrivastav Tulsidas G^*; Chaube Shail K; Kariya Kiran P; Bhanot Sana; Singh Rita; Kumar Dinesh

来源：Journal of Immunoassay and Immunochemistry, 2010, 31(4): 266-278.

DOI：10.1080/15321819.2010.524857

• dehydroepiandrosterone
• heterologous
• homologous
• immunoassay
• site heterology

Homologous and heterologous combinations of enzyme conjugate with immunogen in steroid enzyme immunoassay (EIA) influence labeled steroid recognition by an antibody that affects the sensitivity of the assay. To develop dehydroepiandrosterone (DHEA) enzyme linked immunosorbent assay (ELISA), antibodies were generated against dehydroepiandrosterone-17-carboxymethyloxime-bovine serum albumin (DHEA-17-CMO-BSA) and dehydroepiandrosterone-7-carboxymethyloxime- bovine serum albumin (DHEA-7-CMO-BSA). Two dehydroepiandrosterone (DHEA) horse radish peroxidise (HRP) enzyme conjugates were prepared using two dehydroepiandrosterone derivatives (DHEA-17-CMO and DHEA-7-CMO). Four combinations of homologous and heterologous assays were evaluated. The use of heterologous combination improved the sensitivity of the assay.

• 出版日期2010