Differential proteins expression between gastric cancer and normal cell lines

作者：Zang Jing; Wang Ping; Gao Sanyou; Xiao Di; Zhang Jianzhong; Wang Kaijuan^*

来源：Life Science Journal-Acta Zhengzhou University Overseas Edition, 2008, 5(4): 28-32.

• gastric carcinoma
• two-dimensional electrophoresis
• proteomics

Objective. In search of novel mechanisms leading to the development of gastric cancer, we analyzed differences of protein expression pattern between gastric cancer cell lines of MGC80-3, BGC-823, SGC-7901, AGS and normal gastric epithelial cell line GES-1. Methods. Cells were cultured in vitro and analyzed by proteomics approach. Results. Twenty-two different expression proteins were detected and identified. Nine of them were up-regulated, 10 of them were down-expressed in gastric cancer cells, and 3 of them only expressed in normal gastric epithelial cell. Conclusion. The results suggest that expressed proteins are different between gastric cancer cells and normal cells. These identified proteins may be important in gastric cancer formation and represent potential targets for tumor markers. [Life Science Journal. 2008; 5(4): 28-32] (ISSN: 1097-8135).

• 出版日期2008
• 单位河南省疾病预防控制中心; 中国疾病预防控制中心; 中国疾病预防控制中心传染病预防控制所; 郑州大学