Non-viral gene delivery carrier of probe type host molecule - Interactions between DNA and beta-cyclodextrin derivative complexes (I)

作者：Liu T; Chen L; Hou S; Xue YL; Feng XZ^*

来源：Chinese Science Bulletin, 2006, 51(5): 530-535.

DOI：10.1007/s11434-006-0530-9

• phenanthroline-beta-cyclodextrin derivatives (DZY-1)
• lambda-DNA
• non-virus gene delivery carrier
• agarose gel electrophoresis
• scanning electron microscope (SEM)

A host type non-virus gene delivery carrier, phenanthroline-p-cyclodextrin derivative host molecule, was produced which can be used as molecular probe. Interactions between DZY-1 and DNA were investigated by electrophoresis assay. Hind III enzyme inhibition assay was carried out using DNA condensates induced by host molecules or host-guest molecule complexes to explore their ability to inhibit enzyme digestion. Micro-structure of DNA condensates induced by host molecules and host-guest molecule complexes was observed by scanning electron microscope (SEM). Our work indicates the delivery mechanism of DZY-1 used as a gene delivery carrier and also provides a method to design and produce non-virus gene delivery carriers.

• 出版日期2006-3
• 单位南开大学