MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles

作者：Han Shanhua; Han Huijing; Moon Myeong Ju; Heo Suk Hee; Lim Hyo Soon; Park In Kyu; Cho Chong Su; Kwak Sang Hyun; Jeong Yong Yeon^*

来源：Molecular Imaging and Biology, 2013, 15(6): 685-692.

DOI：10.1007/s11307-013-0643-x

• Sepsis
• Neutrophils
• Cell labeling
• MR imaging
• Neutrophils
• SPION

The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION). %26lt;br%26gt;Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group. %26lt;br%26gt;The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils in vitro. %26lt;br%26gt;The in vitro labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.

• 出版日期2013-12