A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil

作者：Moraes Paula M; Santos Felipe A; Padilha Cilene C F; Vieira Jose C S; Zara Luiz F; Padilha Pedro de M^*

来源：Biological Trace Element Research, 2012, 150(1-3): 195-199.

DOI：10.1007/s12011-012-9502-x

• Metallomics study
• Mercury in fish
• 2D PAGE
• SR XRF

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 +/- 0.7 and 19.8 +/- 0.5 kDa and isoelectric points of 5.6 +/- 0.2 and 7.5 +/- 0.3, respectively.

• 出版日期2012-12