Assessment of Re-188 marked anti MHC class II antibody by peripheral blood mononuclear cells stimulated by donor alloantigen

作者：Ding Guo-ping; Cao Li-ping^*; Liu Jie; Liu Da-ren; Que Ri-sheng; Zhu Lin-hua; Zhou Yi-ming; Mao Ke-jie; Hu Jun-an

来源：Chinese Medical Journal, 2011, 124(16): 2512-2516.

DOI：10.3760/cma.j.issn.0366-6999.2011.16.021

• MHC class II antibody
• dendritic cells
• cytokine
• Re-188
• acute rejection

Background Previous studies showed that anti MHC-II monoclone antibody (MAb) only had partial inhibiting effect of alloreactive mixed lymphocyte reaction (MLR) in vitro and it was unsteady and non-persistent. The aim of this research was to determine whether radioactive isotope Re-188 marked MHC-II antibody could benefit the allograft acceptance in transplantation as compared to normal MHC-II antibody.
Methods Re-188 was incorporated to 2E9/13F(ab')(2) which is against swine MHC class II antigen (MAb-Re-188). Porcine peripheral blood mononuclear (PBMC) cells were examined for proliferation and cytokine mRNA expression after stimulation with MHC-II MAb or MAb-Re-188.
Results The proliferative response of recipient PBMCs in mixed lymphocyte reaction (MLR) to donor alloantigen showed that the stimulation index of MAb-Re-188 group was significantly lower than the MHC-II MAb group and control (P <0.05). mRNA expression of interleukin 2, interferon y and tumor necrosis factor a (type 1 cytokines) was lower in mAb-Re-188 group than the MHC-II MAb group, while interleukin 10 (type 2 cytokines) was higher in MAb-Re-188 group in the first 24 hours.
Conclusion MAb-Re-188 could help the graft acceptance by inhibiting T cell proliferation, lowering the expression of type 1 cytokines and elevating the type 2 cytokines produced by PBMC. Chin Med J 2011;124(16):2512-2516

• 出版日期2011-8-20
• 单位浙江大学