Multimodal nonlinear microscopy of head and neck carcinoma - toward surgery assisting frozen section analysis

作者：Heuke Sandro; Chernavskaia Olga; Bocklitz Thomas; Legesse Fisseha Bekele; Meyer Tobias; Akimov Denis; Dirsch Olaf; Ernst Guenther; von Eggeling Ferdinand; Petersen Iver; Guntinas Lichius Orlando; Schmitt Michael; Popp Juergen

来源：Head and Neck-Journal for the Sciences and Specialties of the Head and Neck, 2016, 38(10): 1545-1552.

DOI：10.1002/hed.24477

<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Treatment of early cancer stages is deeply connected to a good prognosis, a moderate reduction of the quality of life, and comparably low treatment costs.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Head and neck squamous cell carcinomas were investigated using the multimodal combination of coherent anti‐Stokes Raman scattering (CARS), two‐photon excited fluorescence (TPEF), and second‐harmonic generation (SHG) microscopy.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>An increased median TPEF to CARS contrast was found comparing cancerous and healthy squamous epithelium with a <jats:italic>p</jats:italic> value of 1.8·10<jats:sup>−10</jats:sup>. A following comprehensive image analysis was able to predict the diagnosis of imaged tissue sections with an overall accuracy of 90% for a 4‐class model.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Nonlinear multimodal imaging is verified objectively as a valuable diagnostic tool that complements conventional staining protocols and can serve as filter in future clinical routine reducing the pathologist's workload.

• 出版日期2016-10