Mechanism and kinetics of degrading aflatoxin B1 by salt tolerant Candida versatilis CGMCC 3790

作者：Li, Jianlong; Huang, Jun; Jin, Yao; Wu, Chongde; Shen, Dazhan; Zhang, Suyi; Zhou, Rongqing^*

来源：Journal of Hazardous Materials, 2018, 359: 382-387.

DOI：10.1016/j.jhazmat.2018.05.053

• Candida versatilis
• AFB(1)
• LC/TOF-MS
• Degradation products
• Bioremediation

Four products were identified by liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS) for the degradation of aflatoxin B1 (AFB1) is by salt tolerant Candida versatilis CGMCC 3790 (C. versatilis CGMCC 3790), includingII(C14H10O4), III (C14H12O3), IV (C13H12O2), V (C11H10O4), which were not toxic. Based on these products, it is speculated that AFB1 degradation has two pathways. The degradation ratio of active cell component (69.40%) and intracellular component (64.99%) was significantly higher than extracellular component (29.61%), suggesting that the AFB1 removal mainly resulted from biodegradation. The optimal degradation conditions of AFB1 (20 ng/mL) were: incubated at pH 5.0, 25 degrees C for 60 min in liquid medium system. The degradation ratio was ranged from 41.23% similar to 100% at 10.26 similar to 130.44 ng/g in an actual system. This is the first report revealing that a salt tolerant yeast could effectively degrade AFB1. Therefore, Candida versatilis CGMCC 3790 might be an excellent candidate for bioremediation and detoxification for oriental fermentation condiment process.

• 出版日期2018-10-5
• 单位四川大学