Scanning electrochemical microscopy for study of aptamer-thrombin interfacial interactions on gold disk microelectrodes

作者：Bai Huei Yu; Javier del Campo F; Tsai Yu Chen^*

来源：Journal of Colloid and Interface Science, 2014, 417: 333-335.

DOI：10.1016/j.jcis.2013.11.050

• Scanning electrochemical microscope
• Microelectrode
• Thrombin
• Aptamer

A feasibility for the determination of thrombin on gold disk microelectrodes (GDMs) using scanning electrochemical microscopy (SECM) is reported. The assembly process step-by-step of thrombin aptasensor on GDMs is monitored by SECM. SECM analysis reveals the immobilization of thrombin aptamers on GDMs. The interaction between thrombin aptamers and thrombin on GDMs is imaged by SECM with feedback mode using ferrocenemethanol as an electrochemical mediator. The formation of thrombin/thrombin aptamer complex on GDMs results in a decrease in the tip peak current on spatial SECM images. This method is able to linearly and selectively detect thrombin over a linear range from 10(-12) to 10(-5) M with a detection limit of 6.07 fM.

• 出版日期2014-3-1