Majority of CD4(+) T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule

作者：Josefsson Lina^*; King Martin S; Makitalo Barbro; Brannstrom Johan; Shao Wei; Maldarelli Frank; Kearney Mary F; Hu Wei Shau; Chen Jianbo; Gaines Hans; Mellors John W; Albert Jan; Coffin John M; Palmer Sarah E

来源：Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(27): 11199-11204.

DOI：10.1073/pnas.1107729108

Neither the number of HIV-1 proviruses within individual infected cells in HIV-1-infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4(+) T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4(+) T cells and plasma implies ongoing exchange between these compartments both early and late after infection.

• 出版日期2011-7-5