A beta 1-42 regulates astrocytes through JNK/AP-1 pathway

作者：Li, G. -Q.; Cong, D. -W.; Sun, P.; Meng, X.^*

来源：European Review for Medical and Pharmacological Sciences, 2018, 22(7): 2015-2021.

• A beta(1-42)
• Astrocytes
• JNK/AP-1 pathway
• Apoptosis

OBJECTIVE: In A lzheimer's disease (AD), astrocytes are generally found in the surrounding of senile plaques participating in the production of phagocytosis and the removal of toxic compounds such as A beta. This study aimed at investigating the effect of A beta 1-42 on astrocytes.
MATERIALS AND METHODS: Cellular viability of primary cultured astrocytes was analyzed using CCK-8 assay. Quantitative Real-time PCR was used to assess the mRNA expression of JNK and AP-1. The proteins of JNK/AP-1 pathway were investigated using Western blot.
RESULTS: Our findings showed that A beta 1-42 inhibited cell viability and promoted apoptosis in astrocytes in primary culture. Additionally, A beta 1-42 increased the mRNA expression level of AP-1, but had no effect on the expression of JNK. Furthermore, A beta 1-42 increased the protein expression of p-JNK, p-c-jun and Fra-1 and the ratio of p-c-jun/c-jun and p-JNK/JNK.
CONCLUSIONS: We showed that A beta 1-42 promoted cell apoptosis in astrocytes in primary culture. Furthermore, A beta 1-42 activated JNK/AP1 pathway through promoting the phosphorylation of JNK, c-jun and Fra-1 expression, then inducing cell apoptosis.

• 出版日期2018-4
• 单位烟台毓璜顶医院; 青岛大学; 滨州医学院