A HIGH PERFORMANCE METHOD FOR THERMODYNAMIC STUDY ON THE BINDING OF HUMAN SERUM ALBUMIN WITH ERBIUM CHLORIDE

作者：Behbehani G Rezaei^*; Divsalar A; Saboury A A; Faridbod F; Ganjali M R

来源：Journal of Thermal Analysis and Calorimetry, 2009, 96(2): 663-668.

DOI：10.1007/s10973-008-9385-4

• erbium(III) chloride
• human serum albumin
• isothermal titration calorimetry

Thermodynamics of the interaction between erbium(III) chloride, Er(3 ), with human serum albumin (HSA), was investigated at pH 7.0 and in phosphate buffer by isothermal titration calorimetry. Our recently, solvation model was used to reproduce the enthalpies of HSA interaction by Er(3 ) over a broad range of metal ion concentration. The solvation parameters recovered from our new model, attributed to the structural change of HSA and its biological activity. The binding parameters for the interaction of Er(3 ) and HSA indicate that the concentrations of Er(3 ) have no significant effects on the structure of HSA.

• 出版日期2009-5