Brain Arachidonic Acid Cascade Enzymes are Upregulated in a Rat Model of Unilateral Parkinson Disease

作者：Lee Ho Joo; Bazinet Richard P; Rapoport Stanley I; Bhattacharjee Abesh Kumar^*

来源：Neurochemical Research, 2010, 35(4): 613-619.

DOI：10.1007/s11064-009-0106-6

• 6-OHDA
• Cyclooxygenase-2
• Parkinson disease
• Cytosolic phospholipase A(2)
• Rat
• Arachidonic acid
• Cascade
• Signaling
• Dopamine
• Upregulation
• Unilateral
• Asymmetry

Arachidonic acid (AA) signaling is upregulated in the caudate-putamen and frontal cortex of unilaterally 6-hydroxydopamine (6-OHDA) lesioned rats, a model for asymmetrical Parkinson disease. AA signaling can be coupled to D(2)-like receptor initiated AA hydrolysis from phospholipids by cytosolic phospholipase A(2) (cPLA(2)) and subsequent metabolism by cyclooxygenase (COX)-2. In unilaterally 6-OHDA-and sham-lesioned rats, we measured brain expression of cPLA(2), other PLA(2) enzymes, and COX-2. Activity and protein levels of cPLA(2) were significantly higher as was COX-2-protein in caudate-putamen, frontal cortex and remaining brain on the lesioned compared to intact side of the 6-OHDA lesioned rats, and compared to sham brain. Secretory sPLA(2) and Ca(2 ) independent iPLA(2) expression did not differ between sides or groups. Thus, the tonically increased ipsilateral AA signal in the lesioned rat corresponds to upregulated cPLA(2) and COX-2 expression within the AA metabolic cascade, which may contribute to symptoms and pathology in Parkinson disease.

• 出版日期2010-4