Antifungal effects of Lavandula binaludensis and Cuminum cyminum essential oils against Candida albicans strains isolated from patients with recurrent vulvovaginal candidiasis

作者：Minooeianhaghighi M H; Sepehrian L; Shokri H^*

来源：Journal de Mycologie Medicale, 2017, 27(1): 65-71.

DOI：10.1016/j.mycmed.2016.09.002

• Recurrent vulvovaginal candidiasis
• Lavandula binaludensis
• Cuminum cyminum
• Essential oil
• Antifungal activity
• GC-MS

Objective. - Recurrent vulvovaginal candidiasis (RWC), which affects approximately 5% of women of reproductive age, is defined as 4 or more episodes of symptomatic Candida vaginitis within a year. The purposes of this study were to determine the chemical compositions and antifungal susceptibility of Cuminum cyminum (C. cyminum) and Lavandula binaludensis (L. binaludensis) essential oils and their combination against Candida albicans (C. albicans) strains isolated from patients with RWC.
Materials and methods. - C. albicans isolates were identified via germ tube test, CHROMagar and RapID Yeast Plus System. The essential oils were obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas chromatography-mass spectroscopy (GC-MS). The broth micro dilution method was used as antifungal susceptibility test (CLSI-M27-A3).
Results. - The GC-MS analysis allowed 13 components to be determined; the main components of C. cyminum and L. binaludensis essential oils were gamma-terpinene (21.07%) and 1,8-cineole (71.56%), respectively. L. binaludensis and C. cyminum oils were effective in inhibiting C. albicans growth at mean concentrations of 7.91 1.61 mu g/mL and 8.00 +/- 1.89 mu g/mL, respectively. In addition, the combination of C. cyminum with L. binaludensis oils were more active causing inhibition in all C. albicans isolates, with concentrations varying from 3.90 to 11.71 mu g/mL (mean value: 7.22 +/- 1.69 mu g/mL).
Conclusion. - The results suggested the potential substitution of the antifungal chemicals by C. cyminum and L. binaludensis essential oils as natural inhibitors to control the growth of the most important pathogenic Candida species and alternative therapies for RWC.

• 出版日期2017-3