科研之友
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摘要
Background: Repeated elevation of intramuscular pressure (IMP) causes skeletal-muscles damage. The purpose of this paper was to investigate the regenerative effect of satellite cells autografted to the damaged muscle in a rabbit model. Materials and Methods: Twenty-four adult rabbits were randomized into three groups: 1) experimental group, 2) non-graft group, and 3) control group. The model of limb interval compression for 2 hours twice a day for 14 days was established in the experimental and non-graft group. Transplantation was performed in the experimental group and control group. Satellite cells from a half of soleus muscles in the experimental group and control group were isolated and then expanded in vitro. DAPI-tag satellite cells were transplanted back to the remaining half of the soleus muscle. The number of satellite cells with DAPI-tag was determined by fluorescence after grafts. The histological changes were compared at the time of the last compression and at the end of the 28th day after grafting. Results: At the time period of 28 days following grafting of satellite cells into the soleus muscle, the satellite cells from the compressed soleus muscle increased significantly predominantly experimental group (p = 0.013), whereas those in the control group remained the same (p = 0.076). In HE staining, a large cluster of myofibers and interstitial fibers was present in compressed muscle in both the experimental group and non-graft group, while the skeletal muscle fibers and interstitial fibers were in continuity in the control group. After grafting, the muscle showed a great repair but the non-graft muscle exhibited dominant fibrosis. Conclusion: Autografting satellite cells by means of a small amount of expansion in vitro could improve the regenerative efficiency to repair large clusters of the damaged myofibers induced by repeated compression. Clinical Relevance: This technique may be applicable in human compartment syndrome.
- 出版日期2010-8
- 单位上海交通大学
