H19 long noncoding RNA controls the mRNA decay promoting function of KSRP

作者：Giovarelli Matteo; Bucci Gabriele; Ramos Andres; Bordo Domenico; Wilusz Carol J; Chen Ching Yi; Puppo Margherita; Briata Paola^*; Gherzi Roberto

来源：Proceedings of the National Academy of Sciences, 2014, 111(47): E5023-E5028.

DOI：10.1073/pnas.1415098111

• long noncoding RNA
• mRNA decay
• RNA-binding proteins

Long noncoding RNAs (lncRNAs) interact with protein factors to regulate different layers of gene expression transcriptionally or posttranscriptionally. Here we report on the functional consequences of the unanticipated interaction of the RNA binding protein K homology-type splicing regulatory protein (KSRP) with the H19 lncRNA (H19). KSRP directly binds to H19 in the cytoplasm of undifferentiated multipotent mesenchymal C2C12 cells, and this interaction favors KSRP-mediated destabilization of labile transcripts such as myogenin. AKT activation induces KSRP dismissal from H19 and, as a consequence, myogenin mRNA is stabilized while KSRP is repurposed to promote maturation of myogenic microRNAs, thus favoring myogenic differentiation. Our data indicate that H19 operates as a molecular scaffold that facilitates effective association of KSRP with myogenin and other labile transcripts, and we propose that H19 works with KSRP to optimize an AKT-regulated posttranscriptional switch that controls myogenic differentiation.

• 出版日期2014-11-25
• 单位河北医科大学