ALTERATIONS IN ARGINASE-NO-SYNTHASE SYSTEM OF SPERMATOZOA IN HUMAN SUBJECTS WITH DIFFERENT FERTILITY POTENTIAL

作者：Fafula Roman V^*; Iefremova Ulyana P; Onufrovych Olena K; Maksymyuk Hanna V; Besedina Anna S; Nakonechnyi Iosyf A; Vorobets Dmytro Z; Vorobets Zinoviy D

来源：Journal of Medical Biochemistry, 2018, 37(2): 134-140.

DOI：10.1515/jomb-2017-0049

• NO-synthase
• arginase
• male infertility
• pathospermia

Background: Infertility is an important worldwide problem which affects 10-15% of couples globally. Altered NO production has also been implicated in the pathogenesis of the male infertility. The present study was designed to evaluate the changes in the activity of NO-synthase (NOS) and arginase in spermatozoa of patients with infertility.
Methods: The total NOS, Ca2+-dependent constitutive (cNOS) and Ca2+-independent inducible (iNOS) activity and arginase activity were assessed in sperm cells of patients with different forms of pathospermia.
Results: We found a significant increase in iNOS activity, but significantly decreased cNOS and arginase activity in sperm cells of infertile men vs fertile, normozoospermic men (p<0.001). The arginase/NOS ratio significantly de creased compared to control group. The iNOS/cNOS ratio was drastically increased in patients with decreased fertility potential indicating predominance of iNOS. Men with leukocytospermia were distinguished to have the most express iNOS activity.
Conclusions: These observations provide evidence for a disturbed balance between the L-arginine metabolic pathways in sperm cells of infertile men. This imbalance includes the considerable activation of the inducible isoform of NO-synthase accompanied by significant inhibition of its constitutive isoform which indicates disturbances in NO production. In patients with decreased fertility potential the arginase/NOS was shifted towards predominance of iNOS-derived NO production.

• 出版日期2018-4