NHE-1 and beta 1 integrin-dependent monocyte adhesion and migration after glucose, insulin or PPAR gamma stimulation

作者：Zolota Zacharoula; Koliakos George^*; Paletas Konstantinos; Kaloyianni Martha

来源：Cell Adhesion and Migration, 2011, 5(3): 258-265.

DOI：10.4161/cam.5.3.14534

• monocytes
• atherosclerosis-related functions
• cell adhesion
• cell migration
• integrins
• rosiglitazone
• glucose
• insulin
• laminin-1
• collagen type IV
• endothelial cells

In the present study the effect of high glucose concentrations, insulin, PPAR gamma activators (rosiglitazone) and NHE-1 inhibitors (cariporide) in atherosclerosis-related functions of human monocytes was investigated. Monocyte adhesion to laminin-1, collagen type IV and endothelial cells, as well as monocyte migration through the same substrates were studied. Incubation of the monocyte suspension with high glucose concentrations, insulin and rosiglitazone induced all the studied atherosclerosis-related functions of the monocytes. In all these functions the addition of cariporide counteracted the activity of glucose, insulin and rosiglitazone. The use of antigen for beta 1 integrin also counteracted the activity of the above in monocyte adhesion in all three substrates. The data of the present study suggests that PPAR gamma activation in monocytes induces atherosclerosis, and that NHE-1 and beta 1 integrin play an important role in the beginning of atherosclerosis.

• 出版日期2011-6