Depletion of IL-22 during culture enhanced antigen-driven IFN-gamma production by CD4(+)T cells from patients with active TB

作者：Qiu, Yueqin; Huang, Yu; Chen, Jianbo; Qiao, Dongjuan; Zeng, Gucheng; Cai, Jiye^*

来源：Immunology Letters, 2013, 150(1-2): 48-53.

DOI：10.1016/j.imlet.2012.12.008

• IFN-gamma
• IL-22
• Tuberculosis
• CD4(+)T cells

In this study, we mainly investigated the expression of IFN-gamma and IL-22-producing CD4(+)T cells, the relationship between the two cytokines in human tuberculosis. We showed that IFN-gamma and IL-22 were induced by human mycobacterial infection. Besides, CD4(+)T cells expressing IFN-gamma and IL-22 were significantly elevated following PPD stimulation. In addition, IFN-gamma and IL-22 -expressing CD4(+)T cells were distinct from each other. We also found that there was a reciprocal relationship between IFN-gamma and IL-22 production. The production of IL-22 was markedly enhanced in the absence of IFN-gamma, also, the expression of IFN-gamma was increased when the IL-22 signaling was inhibited by monoclonal anti-IL-22 mAb. Furthermore, data revealed that the detectable IL-22 affected the phynotype of IFN-gamma(+)CD4(+)T cells. IL-22 induced upregulation of CD27 expression in IFN-gamma(+)CD4(+)T cells, which might further confirm that IL-22 had an effect on the response of IFN-gamma. Thus, this work provides a previous unknown relationship between IFN-gamma and IL-22 in human TB disease.

• 出版日期2013-2
• 单位深圳市第三人民医院; 广州中医药大学; 暨南大学; 中山大学