A Novel Tri-Protein Bio-Interphase Composed of Cytochrome c, Horseradish Peroxidase and Concanavalin A: Electron Transfer and Electrocatalytics

作者：Song, Yonghai^*; Wang, Yu; Liu, Hongyu; Wang, Li

来源：International Journal of Electrochemical Science, 2012, 7(11): 11206-11218.

• Electron transfer
• Concanavalin A
• Horseradish peroxidase
• Cytochrome c
• H2O2

A tri-protein bio-interphase composed of concanavalin A (Con A), cytochrome c (Cyt c) and horseradish peroxidase (HRP) was developed by layer-by-layer assembly of HRP, Con A, mixture of Cyt c and HRP. The assembly uses lectin-sugar biospecific interactions between Con A and HRP on biocompatible 11-mercaptoundecanoic acid-6-mercapto-1-hexanol modified gold electrode. This novel tri-protein bio-interphase is used as a model system to mimic the electron transfer and electrocatalytic performance of the proteins in living organisms. The tri-protein bio-interphase was characterized by the atomic force microscopy, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The fast electron transfer rate at the bio-interphase indicated synergistic interactions between the involved proteins. Moreover, due to the good electrocatalytic response the tri-protein bio-interphase can be used as hydrogen peroxide (H2O2) and oxygen (O-2) sensor. This study also provides new insights for the electron transfer of multi-proteins in a bio-interphase and the development of biosensors.

• 出版日期2012-11
• 单位江西师范大学