Microwell Chips for Selection of Bio-macromolecules that Increase the Differentiation Capacities of Mesenchymal Stem Cells

作者：Hsu Shan hui^*; Ni Yu Hui; Lee Yung Chun

来源：Macromolecular Bioscience, 2013, 13(8): 1100-1109.

DOI：10.1002/mabi.201200472

• biopolymers
• chitosan
• mesenchymal stem cells (MSCs)
• microwell chips
• spheroids

Microwell chips (25mmx25mm) are fabricated to select proper substrates for growing three-dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio-macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (approximate to 10(5)) are needed for each chip. The expression level of N-cadherin, a cell-adhesion molecule, is used as an indicator for cell-cell interactions. MSC spheroids expressing the highest N-cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio-macromolecules that enhance the differentiation potential of MSCs.

• 出版日期2013-8