A partially methylated mannogalactan from hybrid mushroom pfle 1p: purification, structural characterization, and study of immunoactivation

作者：Maity Prasenjit; Pattanayak Manabendra; Maity Saikat; Nandi Ashis K; Sen Ipsita K; Behera Birendra; Maiti Tapas K; Mallick Pijush; Sikdar Samir R; Islam Syed S^*

来源：Carbohydrate Research, 2014, 395: 1-8.

DOI：10.1016/j.carres.2014.06.004

• Hybrid mushroom
• Heteropolysaccharide
• NMR analysis
• Immunostimulation

A new water-soluble heteropolysaccharide (PS-II) with apparent molecular weight similar to 1.65 x 10(5) Da, was isolated from the fruiting bodies of hybrid mushroom pfle 1p by hot aqueous extraction. It is composed of D-mannose, D-galactose, and 3-O-Me-D-galactose in a molar ratio of 1.0:0.99:1.1. The structural investigation of PS-II has been carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and 1D/2D NMR experiments. Based on the results of these experiments, it was established that PS-II contained a main chain of (1 -> 6) linked alpha-D-galactopyranosyl residues, one of which was substituted at C-2 by a terminal mannopyranosyl residue and also methylated at C-3 position. This heteropolysaccharide (PS-II) exhibited macrophage activation by NO production as well as in vitro splenocyte and thymocyte stimulation.

• 出版日期2014-8-18