Na+-Ca2+ exchange in the regulation of cardiac excitation-contraction coupling

作者：Reuter H^*; Pott C; Goldhaber JI; Henderson SA; Philipson KD; Schwinger RHG

来源：Cardiovascular Research, 2005, 67(2): 198-207.

DOI：10.1016/j.cardiores.2005.04.031

• Na/Ca-exchanger
• e-c coupling
• membrane current
• heart failure
• Ca-channel

Cardiac sarcolemmal Na+-Ca2+ exchange is a central component of Ca2+ signaling essential for Ca2+ extrusion and contributing to a variable degree to the development of the systolic Ca2+ transient. Reports on differential gene expression of Na+-Ca2+ exchange in cardiac disease and the regulation of its thermodynamic equilibrium depending on intracellular gradients of ion concentrations between subcellular compartments have recently put a new complexion on Na+-Ca2+ exchange and its implications for excitation -contraction (E-C) coupling. Heart failure models and genetic approaches to regulate expression of the Na+-Ca2+ exchanger have improved our knowledge of exchanger function. Modest overexpression of the Na+-Ca2+ exchanger in heterozygous transgenic mice had minimal effects on E-C coupling and cardiac function. However, higher levels of Na+-Ca2+ exchange expression in homozygotes led to pathological hypertrophy and failure with an increased interaction between the L-type Ca2+ current and Na+-Ca2+ exchange and reduced E-C coupling gain. These results suggested that the Na+-Ca2+ exchanger is capable of modulating sarcoplasmic Ca2+ handling and at high expression levels may interact with the gating kinetics of the L-type Ca2+ current by means of regulating subsarcolemmal Ca2+ levels. Despite being a central component in the regulation of cardiac E-C coupling, a newly generated mouse model with cardiac-specific conditional knock-out of the Na+-Ca2+ exchanger is viable with unchanged Ca2+ dynamics in adult ventricular myocytes. Cardiac myocytes adapt well to knock-out of the exchanger, apparently by reducing transsarcolemmal fluxes of Ca2+ and increasing E-C coupling gain possibly mediated by changes in submembrane Ca2+ levels. For E-C coupling in the murine model, which relies primarily on sarcoplasmic Ca2+ regulation, this led to the suggestion that the role of Na+-Ca2+ exchange should be thought of as a Ca2+ buffering function and not as a major Ca2+ transporter in competition with the sarcoplasmic reticulum.

• 出版日期2005-8-1