Development and validation of an ELISA for quantification of soluble IFN-beta receptor: assessment in multiple sclerosis

作者：Orpez Zafra Teresa; Pavia Jose; Pinto Medel Maria J; Hurtado Guerrero Isaac; Rodriguez Bada Jose L; Montanez Elisa Martin; Fernandez Oscar; Leyva Laura; Oliver Martos Begona^*

来源：Bioanalysis, 2015, 7(22): 2869-2880.

DOI：10.4155/bio.15.208

Aim: The soluble isoform of the IFN-beta receptor (sIFNAR2) can bind IFN-beta and modulate its activity, although its role in autoimmune diseases remains unknown. Methods: A recombinant human sIFNAR2 protein was cloned, expressed and purified after which we developed and validated an ELISA for its quantification in human serum. Serum sIFNAR2 were assessed in multiple sclerosis (MS) patients and healthy controls. Results: The ELISA has a dynamic range of 3.9-250 ng/ml and a detection limit of 2.44 ng/ml. Serum sIFNAR2 were significantly lower in untreated-MS patients than in healthy controls. Conclusion: The ELISA is suitable for quantification of sIFNAR2 in serum and should facilitate the study of sIFNAR2 in neuroimmunological diseases such as MS.

• 出版日期2015