Antioxidant activity and anti-proliferative effect of a bioactive peptide from chickpea (Cicer arietinum L.)

作者：Xue Zhaohui; Wen Haichao; Zhai Lijuan; Yu Yanqing; Li Yanni; Yu Wancong; Cheng Aiqing; Wang Cen; Kou Xiaohong^*

来源：Food Research International, 2015, 77: 75-81.

DOI：10.1016/j.foodres.2015.09.027

• Synthetic peptide
• p53
• Antioxidant activity
• Antiproliferative effect
• Molecular docking

Our previous study identified a chickpea peptide (CPe-III) derived from chickpea albumin hydrolysate which exhibited a high antioxidant activity. CPe-III-S peptide corresponding to amino acid sequences of CPe-III (RQSHFANAQP) was synthesized chemically to determine the bioactive activity. The reducing power, hydroxyl radical, DPPH radical, ABTS radical scavenging assay and DCFH-DA assay showed significant dose-dependent activities. The cell viability assay indicated a high inhibition activity on MCF-7 and MDA-MB-231 cells with EC50 values of 2.38 and 1.50 mu mol/mL. The p53 protein increased with increasing concentration of CPe-III-S tested by ELISA Kit. The specific binding of peptide and p53 protein was determined by means of molecular docking. These results indicated that CPe-III-S effectively inhibited the proliferation of breast cancer cells by increasing the p53. This is the first report of the synthetic peptide which based on CPe-III derived from the enzymatic hydrolysate of chickpea to exhibit the antioxidant activity and antiproliferative effect.

• 出版日期2015-11
• 单位天津大学