Protective effect of methylprednisolone on warm ischemia-reperfusion injury in a cholestatic rat liver

作者：Subhas Gokulakkrishna; Gupta Aditya; Bakston Daniel; Silberberg Boris; Lobocki Cathy; Andrus Lee; Decker Melissa; Mittal Vijay K; Jacobs Michael J^*

来源：American Journal of Surgery, 2010, 199(3): 377-380.

DOI：10.1016/j.amjsurg.2009.09.012

• Ischemia-reperfusion injury
• Cholestasis
• Methylprednisolone
• Liver resection

BACKGROUND: Cholestasis has been identified as a risk factor for oxidative stress, and it potentially enhances after ischemic-reperfusion injury. The aim of this study was to evaluate the role of methylprednisolone on warm ischemia-reperfusion injury in the presence of cholestasis.
METHODS: A reversible cholestatic rat model was created. After 7 days, rats received 30 mg/kg of intravenous methylprednisolone 2 hours before ischemia, followed by 30 minutes of ischemia. Rats were euthanized 24 hours after ischemia. Serum aspartate aminotransferase and interleukin-6 were measured, and the liver was harvested for histology and myeloperoxidase estimation.
RESULTS: Methylprednisolone had a protective effect, with a statistically significant decrease in aspartate aminotransferase (P = .01) and a trend toward decreased levels of interleukin-6 (P = .07). Histology showed a significant difference in architectural distortion (P = .01), cytoplasmic vacuolation (P = .01), and nodular hepatocellular necrosis (P = .04).
CONCLUSIONS: Methylprednisolone attenuated the ischemic-reperfusion injury in the presence of cholestasis and can be considered for clinical use in the presence of cholestasis.

• 出版日期2010-3