Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

作者：Hermenean Anca^*; Stan Miruna; Ardelean Aurel; Pilat Luminita; Mihali Ciprian Valentin; Popescu Cristina; Nagy Lajos; Deak Gyoergy; Zsuga Miklos; Keki Sandor; Bacskay Ildiko; Fenyvesi Ferenc; Costache Marieta; Dinischiotu Anca; Vecsernyes Miklos

来源：Open Life Sciences, 2015, 10(1): 225-236.

DOI：10.1515/biol-2015-0017

• Silybum marianum
• seed oil
• CCl4
• oxidative stress
• antioxidant
• hepatoprotection

This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.

• 出版日期2015-1