Activity of ERK regulates mucin 3 expression and is involved in undifferentiated Caco-2 cell death induced by 3-oxo-C-12-homoserine lactone

作者：Shimizu Hidehisa^*; Baba Nanako; Nose Takuma; Taguchi Ryoko; Tanaka Shinya; Joe Ga Hyun; Maseda Hideaki; Nomura Nobuhiko; Hagio Masahito; Lee Ja Young; Fukiya Satoru; Yokota Atsushi; Ishizuka Satoshi; Miyazaki Hitoshi

来源：Bioscience Biotechnology and Biochemistry, 2015, 79(6): 937-942.

DOI：10.1080/09168451.2015.1006570

• quorum sensing
• sepsis
• enterobacterium
• intestine
• autoinducer

The signal molecule, 3-oxo-C-12-homoserine lactone (3-oxo-C-12-HSL), is similar to a mammalian hormone in bacteria. Although most studies have examined the effects of high 3-oxo-C-12-HSL concentrations (>200 mu M) on mammalian cellular functions because ~600 mu M 3-oxo-C-12-HSL can be secreted in biofilms of Pseudomonas aeruginosa grown in vitro, we previously showed that a low 3-oxo-C-12-HSL concentration (30 mu M) induces the apoptosis of undifferentiated Caco-2 cells through suppressing Akt activity. Here, we found that a low concentration of 3-oxo-C-12-HSL-activated ERK1/2 in undifferentiated Caco-2 cells. Incubating cells with the ERK pathway inhibitor U0126 for 30min alleviated the mucin 3 (MUC3) expression suppressed by 3-oxo-C-12-HSL, and the upregulation of MUC3 expression induced by a 48-h incubation with U0126-reduced cell death. Thus, altered MUC3 expression caused by long-term attenuated ERK1/2 activity might correlate with the death of undifferentiated Caco-2 cells induced by 3-oxo-C-12-HSL.

• 出版日期2015-6-3