Analysis of monosomy-3 in immunomagnetically isolated circulating melanoma cells in uveal melanoma patients

作者：Tura Ayseguel^*; Merz Hartmut; Rein**erg Mihaela; Lueke Matthias; Jager Martine J; Grisanti Salvatore; Lueke Julia

来源：Pigment Cell & Melanoma Research, 2016, 29(5): 583-589.

DOI：10.1111/pcmr.12507

• uveal melanoma
• circulating melanoma cells
• monosomy-3
• dual immunomagnetic enrichment
• immuno-FISH

Monosomy-3 in primary uveal melanoma (UM) is associated with a high risk of metastasis and mortality. Although circulating melanoma cells (CMC) can be found in most UM patients, only approximately 50% of the patients develop metastases. We utilized a novel immuno-FISH assay to detect chromosome-3 in intact CMC isolated by dual immunomagnetic enrichment. Circulating melanoma cells were detected in 91% of the patients (n = 44) with primary non-metastatic UM, of which 58% were positive for monosomy-3. The monosomy-3 status of CMC corresponded to the monosomy-3 status of the primary tumor in 10 of the 11 patients where this could be tested. Monosomy-3 in the CMC was associated with an advanced tumor stage (P = 0.046) and was detected in all four patients who developed metastasis within the follow-up period of 4 yr. This non-invasive technique may enable the identification of UM patients at risk for metastasis particularly when a primary tumor specimen is unavailable.

• 出版日期2016-9