Involvement of the NLRC4-Inflammasome in Diabetic Nephropathy

作者：Yuan, Fang; Kolb, Ryan; Pandey, Gaurav; Li, Wei; Sun, Lin; Liu, Fuyou; Sutterwala, Fayyaz S.; Liu, Yinghong^*; Zhang, Weizhou^*

来源：PLos One, 2016, 11(10): e0164135.

DOI：10.1371/journal.pone.0164135

• MONOCYTE CHEMOATTRACTANT PROTEIN-1
• NF-KAPPA-B
• INFLAMMASOME ACTIVATION
• BACTERIAL LIGANDS
• RECEPTOR
• INJURY
• TRANSCRIPTION
• RECOGNITION
• MACROPHAGES
• EXPRESSION

Diabetic nephropathy (DN) is the leading cause of end-stage kidney disease worldwide but current treatments remain suboptimal. The role of inflammation in DN has only recently been recognized. It has been shown that the NLRP3-inflammasome contributes to DN development by inducing interleukin (IL)-1 beta processing and secretion. In an effort to understand other IL-1 beta activating mechanism during DN development, we examined the role of the NLRC4-inflammasome in DN and found that NLRC4 is a parallel mechanism, in addition to the NLRP3-inflammasome, to induce pro-IL-1 beta processing and activation. We found that the expression of NLRC4 is elevated in DN kidneys. NLRC4-deficiency results in diminished DN disease progression, as manifested by a decrease in blood glucose and albumin excretion, as well as preserved renal histology. We further found that DN kidneys have increased F4/80(+) macrophages, increased IL-1 beta production, and other signaling pathways related to kidney pathology such as activation of NF-kappa B and MAP kinase pathways, all of which were rescued by NLRC4-deficiency. This study demonstrates NLRC4-driven IL-1 beta production as critical for the progression of DN, which underscores the importance to target this pathway to alleviate this devastating disease.

• 出版日期2016-10-5
• 单位中南大学