Dispersive Micro-Solid Phase Extraction Combined with High-Performance Liquid Chromatography for the Determination of Three Penicillins in Milk Samples

作者：Yahaya Noorfatimah; Sanagi Mohd Marsin^*; Mitome Takahito; Nishiyama Norikazu; Ibrahim Wan Aini Wan; Nur Hadi

来源：Food Analytical Methods, 2015, 8(5): 1079-1087.

DOI：10.1007/s12161-014-9991-7

• Liquid chromatography
• Penicillins
• Microextraction
• Dispersive micro-solid phase extraction
• Mesoporous carbon
• Milk

A simple, rapid, and environmentally friendly microextraction termed COU-2-D-mu-SPE has been developed for the analysis of three penicillins (i.e., oxacillin, cloxacillin, and dicloxacillin) in milk samples. An ordered mesoporous carbon, COU-2, was synthesized and used as the sorbent in dispersive micro-solid phase for the extraction of selected penicillins prior to high-performance liquid chromatography-ultraviolet detection (HPLC-UV). Several important parameters, namely, pH value, salt addition, desorption solvent, extraction time, desorption time, and amount of COU-2, were investigated and optimized. Under the optimum extraction conditions, the method showed good linearity in the range of 10-5,000 mu g L-1 (r (2) a parts per thousand yenaEuro parts per thousand 0.9994), low limits of detection (2.0-3.3 mu g L-1), acceptable reproducibility (relative standard deviation (RSD) 6.2-8.8 %, n = 9), and satisfactory relative recoveries (80.3-99.5 %) for studied penicillins in milk. The proposed COU-2-D-mu-SPE method has been successfully applied to six commercial milk samples, and the extraction of blank samples indicated that all samples were free from selected penicillin contamination.

• 出版日期2015-5