A Comparison of Microbial Community Function and Structure in Rehabilitated Asbestos and Coal Discard Sites

作者：Claassens Sarina^*; van Ren**urg Peet Jansen; Liebenberg Danica; van Ren**urg Leon

来源：Water, Air, and Soil Pollution, 2012, 223(3): 1091-1100.

DOI：10.1007/s11270-011-0927-1

• Enzymatic activity
• Mine dump rehabilitation
• Multivariate statistical analyses
• Phospholipid fatty acid
• Revegetation

Previous studies have made some progress with the use of microbial community properties as assessment criteria for rehabilitation success of post-mining areas. Currently, there is a need for reference ranges of specific properties in rehabilitated post-mining sites to make this approach more practical. The aim of this investigation was to compare assessment parameters indicative of microbial community enzymatic assays) and structure (phospholipid fatty acid (PLFA) analysis) in rehabilitated asbestos and coal discard sites and to establish ranges of minimum and maximum values for these parameters in both types of sites. The range established for dehydrogenase activity in coal discard sites was 24.3-339.5 mu g INF g(-1) 2 h(-1) and for asbestos 44.5-544.6 mu g INF g(-1) 2 h(-1). Ranges were also established for beta-glucosidase, urease, acid phosphatase and alkaline phosphatase. Complete PLFA profiles were determined and ranges established for major PLFA groups and ratios in both types of discard. From the PLFA profiles, viable microbial biomass was determined as 6,080-29,851 and 8,128-47,242 pmol g(-1) dry weight for the coal and asbestos discard sites, respectively. While similar ranges were observed for both types of discard, a canonical correspondence analysis that accounts for functional and structural characteristics showed that sites clustered according to the origin of the samples.

• 出版日期2012-3
• 单位西北大学