科研之友
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摘要
Active prevalence or canine parvovirus (CPV) was studied in the dog population or Pakistan. A total of 40 fecal samples were taken aseptically from dogs clinically suspected for parvovirus infection. The clogs were showing enteritis, bloody and smelly diarrhea. Each of the samples was processed for hemagglutinating (HA) activity and polymerise chain reaction (PCR) based detection of capsid protein using specific primers against CPV2 and CPV2b. Only 30 samples showed HA titer ranging from I :16 to 1:512. Added to this, only 29 and 27 IIA positive samples affirmed CPV2 and CPV 2h amplification. respectively whereas one IIA positive sample ( I :32) was found negative through PCR. The results suggest that HA is a rapid screening test for diagnosis or CPV infection. The PCR technique is more reliable and rules out the possibility of other hemagglutinating pathogens causing hemorrhagic enteritis in clogs. Further, it confirms that both CPV2 and CPV2b are pathogenic strains prevailing in Pakistan.
- 出版日期2010-12
