摘要

High molecular weight glutenin subunits (HMW-GS) content and glutenin macropolymer (GMP) particle size distribution affect grain quality fatally in wheat. The present study was carried out to evaluate the effect of nitrogen rate on HMW-GS accumulation and GMP particle size distribution in wheat grain. The results showed that the diameter of GMP particles changed in the range of 0.375 similar to 200 mu m. Volume distribution of GMP particles show the typical bimodal distribution with peak values in the ranges of 4 similar to 4.9 mu m and 87.9 similar to 105.9 mu m, respectively. A typical population of number distribution of GMP particles with peak values range from 0.9 to 1 mu m. Most particles in the wheat grain were smaller particles. Nitrogen application could increase the percentages of volume of GMP particles and >100 mu m within the nitrogen rates from 0 to 240 kg ha(-1), which indicates that nitrogen application (240 kg ha(-1)) was conducive to the formation of larger GMP particles. However, excessive nitrogen of 360 kg hm(-2) went against the formation of large particles. The appropriate nitrogen fertilizer could improve the total HMW-GS accumulation, but too much was unfavorable. The appropriate nitrogen rate especially could improve the contents of HMW-GS (Dx5+Dy10) coded by Glu-D locus. Remarkable negative correlation was found between the content of HMW-GS and volume percentage of GMP particles of 12-100 mu m. However, there was a significant positive correlation between the content of HMW-GS and volume percentage of particles >100 mu m. The D(4, 3) increased with decreasing GMP particles >100 mu m. The results suggested that larger GMP particles had more HMW-GS content.

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