Analysis of Lewis antigens on cell surface and alpha 1,3 fucosyl-transferase subtypes in H7721 human hepatocarcinoma cells

作者：Guo P; Zhang Y; Zhang XY; Chen HL^*; Zhang Y; Wang H; Narimatsu H

来源：Journal of Experimental & Clinical Cancer Research, 2003, 22(1): 135-139.

• hepatocarcinoma cell line
• Lewis antigens
• cell adhesion
• migration
• invasion
• fucosyltransferase
• real-time RT-PCR

The expressions of Lewis antigens on H7721 human hepatocarcinoma cells were detected with monoclonal antibodies and flow-cytometry. It was found that H7721 mainly expressed SLe(x), and a small amount of SDLe(x), but Le(x) and SLe(a) was negligible. The monoclonal antibody of SLe(x) (KM93) significantly blocked the adhesion of H7721 cells to human umbilical vein epithelial cells, as well as cell migration and invasion, but the blocking effect of SDLe(x) antibody (FH6) was not statistically significant. The expressions of five subtypes of alpha1,3fucosyl-transferases (alpha1,3FucTs), the enzyme responsible for the fucosylation step in Lewis antigen synthesis, were also studied using real-time RT-PCR. The expression of FucT mRNAs were FucT-IV > FucT-III > FucT-VI greater than or equal to wFucT-VII > FucT-IX. FucT VI is supposed to be the main enzyme responsible for the synthesis SLe(x) and SDLex. FucT-VII and III may also participate in SLe(x) synthesis. The absence of FucT-IX expression and FucT-III not being the rate-limiting enzyme for SLe(a) synthesis may be the reasons for the negligible expressions of Le(x) and SLe(a) on the cell surface, respectively.

• 出版日期2003-3
• 单位复旦大学