Microarray analysis of 1ncRNA and mRNA expression profiles in mice with allergic rhinitis

作者：Ma, Yue; Shi, Le; Zheng, Chunquan^*

来源：International Journal of Pediatric Otorhinolaryngology, 2018, 104: 58-65.

DOI：10.1016/j.ijporl.2017.10.046

• Long non-coding RNA
• Microarray analysis
• CD4*T cell
• Allergic rhinitis
• AR murine model

Objectives: We aimed to identify the effect of lncRNAs in CD4(+) T cells on Allergic rhinitis (AR). @@@ Methods: The present study conducted a microarray to identify the expression profiles of 1ncRNA and mRNA in CD4(+) T cells in both AR murine models and normal controls. And qRT-PCR was used to confirm the results. GO and KEGG enrichment analysis were used to show all related pathways and a co-expression network was conducted to find IncRNAs which have high correlation with these pathways. @@@ Results: The results showed that the two groups contained a total of 158 deregulated lncRNAs, of which 110 were upregulated and 48 were downregulated. And positive regulation of calcium ion transport, B cell activation, chemokine-signaling pathways and calcium-signaling pathways may be involved in the development of T cells in AR pathology. Finally, we can find the differentially expressed mRNA in the pathways related to T cell differentiation correlated with many deregulated IncRNAs. @@@ Conclusions: The present study was the first to show the differential expression profiles of lncRNAs in the CD4 T cells of an AR murine model, which may provide significant insights into AR pathogenesis and offer new treatment targets to alleviate it.

• 出版日期2018-1
• 单位复旦大学