Down-regulation of caffeic acid O-methyltransf erase in maize revisited using a transgenic approach

作者：Piquemal J; Chamayou S; Nadaud I; Beckert M; Barriere Y; Mila I; Lapierre C; Rigau J; Puigdomenech P; Jauneau A; Digonnet C; Boudet AM; Goffner D; Pichon M^*

来源：Plant Physiology, 2002, 130(4): 1675-1685.

DOI：10.1104/pp.012237

Transgenic maize (Zea mays) plants were generated with a construct harboring a maize caffeic acid O-methyltransferase (COMT) cDNA in the antisense (AS) orientation under the control of the maize Adlt1 (alcohol dehydrogenase) promoter. Adhl-driven beta-glucuronidase expression was localized in vascular tissues and lignifying sclerenchyma, indicating its suitability in transgenic experiments aimed at modifying lignin content and composition. One line of AS plants, COMT-AS, displayed a significant reduction in COMT activity (15%-30% residual activity) and barely detectable amounts of COMT protein as determined by western-blot analysis. In this line, transgenes were shown to be stably integrated in the genome and transmitted to the progeny. Biochemical analysis of COMT-AS showed: (a) a strong decrease in Klason lignin content at the flowering stage, (b) a decrease in syringyl units, (c) a lower p-coumaric acid content, and (d) the occurrence of unusual 5-OH guaiacyl units. These results are reminiscent of some characteristics already observed for the maize bm3 (brown-midrib3) mutant, as well as for COMT down-regulated dicots. However, as compared with bm3, COMT down-regulation in the COMT-AS line is less severe in that it is restricted to sclerenchyma cells. To our knowledge, this is the first time that an AS strategy has been applied to modify lignin biosynthesis in a grass species.

• 出版日期2002-12