Additive Impairment of Synaptic Signaling in Cultured Cortical Neurons by Exogenously-Applied Oligomerized Amyloid-beta and Airborne Nanoparticles Generated during Photocopying

作者：Lee Sangmook; Sohal Ikjot S; Therrien Mikaela A; Pal Anoop K; Bello Dhimiter; Shea Thomas B^*

来源：Journal of Alzheimer's Disease, 2015, 47(1): 49-54.

DOI：10.3233/JAD-150099

• Amyloid-beta
• multi-electrode arrays
• nanoparticles
• signaling
• neurotoxicology

Photocopying in offices and printing centers releases nanoparticles that can reach the brain following inhalation. We examined whether subcytotoxic levels of airborne photocopy-emitted nanoparticles could potentiate perturbation of synaptic signaling in cultured neurons following exposure to amyloid-beta (A beta). Signaling was only transiently inhibited by A beta or nanoparticles individually, but remained statistically reduced in cultures receiving both after 24 h. In vitro and in vivo studies with copier emitted nanoparticles have consistently demonstrated inflammation, oxidative stress, and cytotoxicity. Since A beta can accumulate years before cognitive decline, subcytotoxic levels of nanoparticles are one factor that could potentiate A beta-induced impairment of synaptic activity during these early stages.

• 出版日期2015