A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection

作者：Tran Thi Ngoc Dung; Pham Thanh Duy; Sessions October M; Sangumathi Uma K; Voong Vinh Phat; Pham Thi Thanh Tam; Nguyen Thi Nguyen To; Tran My Phuc; Tran Thi Hong Chau; Nguyen Ngoc Minh Chau; Ngoc Nguyen Minh; Thwaites Guy E; Rabaa Maia A^*; Baker Stephen

来源：BMC Genomics, 2017, 18(1): 324.

DOI：10.1186/s12864-017-3714-6

• Rotavirus A
• Genomics
• Phylogenetics
• Genome sequencing
• Reassortment
• Co-infection
• Antibody capture

Background: Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material.
Methods: To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents.
Results: Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30).
Conclusions: This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems.
Trial registration (prospectively registered): Current Controlled Trials ISRCTN88101063. Date of registration: 14/06/2012

• 出版日期2017-4-24