Neutrophil depletion reduces endometriotic lesion formation in mice

作者：Takamura Masashi; Koga Kaori; Izumi Gentaro; Urata Yoko; Nagai Miwako; Hasegawa Akiko; Harada Miyuki; Hirata Tetsuya; Hirota Yasushi; Wada Hiraike Osamu; Fujii Tomoyuki; Osuga Yutaka

来源：American Journal of Reproductive Immunology, 2016, 76(3): 193-198.

DOI：10.1111/aji.12540

• endometriosis
• Gr-1
• mouse model

ProblemNeutrophils are known to be accumulated in endometriosis; however, direct evidence of the impact of neutrophils in the development of endometriosis was lacking. To clarify the importance of neutrophils, we examined the effect of neutrophil depletion on the development of endometriosis.
Method of studyOvariectomized, estradiol-replaced, 8-week-old, female BALB/c mice were injected with endometrial fragments (Day 0). Neutrophils were depleted by anti-Gr-1 antibody, either in the early stage (from Day 1 to Day 3, group E) or late stage (from Day 8 to Day 12, group L). Control mice (group C) did not receive antibodies. On Day 14, mice were killed and the number and weight of endometriotic lesions were counted and weighed.
ResultsThe number of endometriotic lesions was significantly less in group E in comparison with Group C and Group L. Weight per lesion did not differ between groups.
ConclusionNeutrophils are essential for the initial formation of endometriosis.

• 出版日期2016-9